Frequency of CCR5-delta32 Mutant Allele in Sudanese Patients with Sickle Cell Anemia

Background: Sickle cell anemia (CSA) is one of a group of hemoglobin disorders known as sickle cell disease in which the sickle β-globin gene is inherited. The pathophysiology of sickle cell disease (SCD) is based on the chronic hemolysis, vaso-occlusive episodes, infection and chronic inflammatory conditions. The CCR5 gene which encodes CCR5,Th5,cell associated chemokine receptor act as pro-inflammatory mediator, the presence of mutant allele known as CCR5 delta 32 makes it non- functional, and lower inflammatory picture. Thus it could confer a selective advantage on patient with sickle cell disease because it induce less efficientTh1 response (decrease inflammation and morbidity) its effect on the inflammatory response and morbidity in patients with sickled cell disease.

Objective: This study aimed at the detection of the frequency of CCR5delta 32 polymorphism among Sudanese patients with SCA.

Materials and Methods: This is a case control study, conducted in Alneelain University –khartoum state during the period from August to December 2018. A total of study population, 60 participants (30 patients with SCA and 30 normal controls), were enrolled in this study. 2.5 milliliter of EDTA anticoagulated blood was collected from each subject. DNA was extracted by salting out method, and target DNA regions of the CCR5 delta 32 gene were amplified using allele specific polymerase chain reaction (AS-PCR).

Results: The frequency of CCR5 delta32 polymorphism in study populations was 0%.

Conclusion: There is no any CCR5 delta32 among Sudanese patients with sickle cell anemia.