Rizk, Mohammed Salem and Sultan, Maha and Habib, Ibrahim Hassan and Mohamed, Dalia and Tony, Rehab Moussa (2016) Simultaneous Spectrophotometric Determination of Paracetamol and Dantrolene Sodium by Chemometric Methods. British Journal of Pharmaceutical Research, 13 (3). pp. 1-8. ISSN 22312919
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Abstract
Aim: Simultaneous determination of Paracetamol (PAR) and Dantrolene Sodium (DAN) in pharmaceutical formulations was carried spectrophotometrically.
Study Design: Two different chemometric techniques namely Partial Least-Squares (PLS) and Classical Least Squares (CLS). In both techniques, a full factorial experimental design was done using three levels and both components in mixtures.
Methodology: This design was used to prepare the concentration data matrix in solvent composed of 2.5 mM NaOH solution. The wavelength range of 220 – 500 nm was chosen with the intervals of Δλ= 1 nm, through which the corresponding absorbance data matrix was measured. Both of the absorbance and concentration data matrices were used to obtain regression. The regression was used for the prediction of the unknown concentrations of PAR and DAN in their mixture. Neither chemical separation steps nor prior graphical treatment of the overlapped spectra was required for this procedure.
Results: The linearity of calibration curve was fulfilled over concentrations of 1-20 and 1.5 – 15 μg/mL for PAR and DAN, respectively.
Conclusion: Validation was done for multivariate methods using both authentic mixtures and pharmaceutical preparations. Assessment of accuracy and precision was done for each method and results were compared.
Item Type: | Article |
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Subjects: | Lib Research Guardians > Medical Science |
Depositing User: | Unnamed user with email support@lib.researchguardians.com |
Date Deposited: | 29 Jun 2023 04:07 |
Last Modified: | 13 Jan 2024 04:43 |
URI: | http://journal.edit4journal.com/id/eprint/1229 |